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cleancap reagent ag  (New England Biolabs)


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    New England Biolabs cleancap reagent ag
    Cleancap Reagent Ag, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleancap reagent ag/product/New England Biolabs
    Average 96 stars, based on 136 article reviews
    cleancap reagent ag - by Bioz Stars, 2026-06
    96/100 stars

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    A. Editing frequencies at the EPOR-sg1 locus measured at Day 4 post-electroporation with CBE6 mRNA prepared under different conditions, including capping strategies (CleanCap AG), incorporation of modified nucleotides (e.g., pseudouridine), RNase inhibitor addition, freeze-thaw cycles, and storage duration. B. Heatmaps showing EPOR -sg1 editing frequencies across varying CBE6 mRNA doses (2-10 µg) and electroporation conditions using Lonza 4D nucleofector with P3 buffer + DZ-100 code or B1mix buffer + CM-137 code. Data are shown as replicate measurements from a single donor (n = 1).

    Journal: bioRxiv

    Article Title: Combinatorial base editing couples disease correction with lineage amplification in hematopoietic stem and progenitor cells

    doi: 10.64898/2026.04.13.718029

    Figure Lengend Snippet: A. Editing frequencies at the EPOR-sg1 locus measured at Day 4 post-electroporation with CBE6 mRNA prepared under different conditions, including capping strategies (CleanCap AG), incorporation of modified nucleotides (e.g., pseudouridine), RNase inhibitor addition, freeze-thaw cycles, and storage duration. B. Heatmaps showing EPOR -sg1 editing frequencies across varying CBE6 mRNA doses (2-10 µg) and electroporation conditions using Lonza 4D nucleofector with P3 buffer + DZ-100 code or B1mix buffer + CM-137 code. Data are shown as replicate measurements from a single donor (n = 1).

    Article Snippet: In vitro transcription (IVT) was performed using the HiScribe T7 High Yield RNA Synthesis Kit or the HiScribe T7 mRNA Kit with CleanCap AG (New England Biolabs; E2040 or E2080).

    Techniques: Electroporation, Modification